<i>dKlf15</i> is critical for nephrocyte development.

Abstract

<p>(A) Schematic of the <i>dKlf15</i> gene. An allele of <i>dKlf15</i>, originally called <i>Bteb2</i>^{<i>f06447</i>}, and now renamed <i>dKlf15</i>^<i>NN</i>, contains a piggyBac insertion (white box) within the coding region. The red bars define the primer sequence used to detect <i>dKlf15</i> expression. (B) <i>dKlf15</i> expression in adult heart from control (<i>CaS</i>) and mutant (<i>dKlf15</i>^<i>NN</i>) flies. (C) Phase contrast micrographs of the heart from wild type (C’) and <i>dKlf15</i>^<i>NN</i> mutant females (C”) overlaid with fluorescence images of a Hoechst-stained adult heart; nuclei are re-coloured black against a white background. Arrows indicate pericardial nephrocytes, HT = heart tube; scale bar = 50 μm. (D) Micrographs of Hoechst-stained larval oesophagus (OE) at the point it meets the proventriculus (PV) in wild type and <i>dKlf15</i>^<i>NN</i> mutants (D’ % D”, respectively); GC = garland cells (arrow); scale bar = 25 μm. (E) Adult heart from wild type (E’), <i>dKlf15</i>^<i>NN</i> mutants (E”) or flies where <i>dKlf15</i> had been silenced using either <i>dot-Gal4</i> or <i>Hand-Gal4</i> (E”‘ & E”“, respectively), stained with wheat-germ agglutinin (red) and phalloidin^FITC (green); arrow indicates pericardial nephrocytes; scale bar = 100 μm. (F) Adult heart stained with antibodies to Amnionless (green) and phalloidin (red); hearts were from female flies heterozygous for the mutant <i>dKlf15</i>^<i>NN</i> allele and a wild type copy of the allele (F’) or heterozygous for the <i>dKlf15</i>^<i>NN</i> mutant allele and a deletion spanning the <i>dKlf15</i> locus (F”; (<i>Df(1)ED6727</i>)); HT = heart tube.</p