Protein levels of TaNAC2L in <i>TaNAC2L-OX</i> transgenic lines.

Abstract

<p>A. TaNAC2L protein and transcript levels in <i>TaNAC2L</i>-overexpressing lines (#2, #9 and #13) under normal conditions and 38°C heat stress. Western blot analyses were performed with the anti-HA monoclonal antibody. B. Seven-day-old wild-type (WT) and <i>TaNAC2L-OX</i>-#13 seedlings were treated at 38°C for 2 h. After heat treatment, seedlings were transferred to dimethylsulfoxide (DMSO) for 0.5, 1, 2, 3, 4, 6, or 8 h; or DMSO with 50 μM MG132 for 1, 3, 4, 6, 8, 12, or 24 h before harvesting. The HA antibody was used for the immunoblot analyses.</p