<p><b>A</b>, Reverse transcription PCR (RT-PCR) on RNA extracted from Oli-<i>neu</i> or IMS32 cells using <i>Mbp</i>-specific primers. The 88nt long amplicon for <i>Mbp</i> was visualized in an ethidium bromide-stained 4% agarose gel. <b>B</b>, Western Blots of lysates from P18 mouse brain (brain lysate), primary oligodendrocytes (pOL, 7DIV), IMS32 and Oli-<i>neu</i> cells using MBP and GAPDH (loading control) specific antibodies. <b>C</b>, Reverse transcription PCR (RT-PCR) on RNA extracted from Oli-<i>neu</i> or IMS32 cells using a sncRNA715-specific primer assays. PCR products (~60-nt long due to the use of hairpin primers in the RT reaction) were visualized in an ethidium bromide stained 4% agarose gel. <b>D</b>, Northern Blots with RNA from IMS32 and undifferentiated primary Schwann cells (pSC) shows expression of sncRNA715 in IMS32 and a lower expression in pSC. Synthetic sncRNA715 (715-mimic) and U6 snRNA were used as positive control and loading control, respectively. <b>E</b>, RT-PCR on RNA from IMS32 and undifferentiated pSC confirms lower expression of sncRNA715 in pSC compared to IMS32 cells shown in D. 715-mimic was used as positive control and snoRNA135 as loading control.</p
