Phenolic profiles and antioxidant capacities of crude extracts and subsequent fractions from <i>Potentilla fruticosa</i> L. leaves
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Abstract
<p>This work aimed to further investigate the phenolic profiles and antioxidant capacities of the crude extracts and the subsequent fractions of <i>Potentilla fruticosa</i> leaves. Result showed that <i>P. fruticosa</i> leaves contained high amounts for hyperoside, ellagic acid and (+)-catechin contents, and the highest amount being registered for hyperoside (17.67 mg g<sup>−1</sup>). Nine sub-fractions were obtained after column chromatographic separation. EF-3, EF-4, EF-5 and BF-2 presented higher values for their total phenolic or flavonoid, (+)-catechin, ellagic acid and hyperoside content. Besides, EF-3, EF-4, BF-2 and BF-3 showed significant <i>in vitro</i> antioxidant capacities and protective effects on <i>Escherichia coli</i> under peroxide stress. The correlation between chromatograms and antioxidant activity showed that (+)-catechin, ellagic acid and hyperoside may play crucial roles in the antioxidant capacities of <i>P. fruticosa</i> and could be used as chemical markers for its quality assessment. Moreover, this is the first time <i>P. fruticosa</i> leaves have been systematically studied.</p