SDS-PAGE analysis of recombinant His-EDDIE-BnPRP1 expressed in <i>E</i>. <i>coli</i> BL 21 (DE3) and Tricine-SDS-PAGE analysis of BnPRP1 renaturation.

Abstract

<p>(A) SDS-PAGE analysis of recombinant His-EDDIE-BnPRP1. Lane M, protein molecular weight maker; Lanes 2, precipitation of His-EDDIE-BnPRP1; Lane 1, <i>E</i>. <i>coli</i> BL 21 (DE3) cells transformed with pET30a plasmid as a control. Lane 3, purified His-EDDIE-BnPRP1. (B) Tricine-SDS-PAGE analysis of BnPRP1 renaturation. Lane M, protein molecular weight marker; Lanes 1, 2, refolded BnPRP1 after 8 h.</p

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