<i>Myb and Cebpa</i> knockdown in primary KIT⁺ enriched bone marrow cells.

Abstract

<p>(A) Two-dimensional flow cytometry dot plot showing the analysis of the KSL compartment of a KIT⁺ enriched population transfected with siRNA control or undergoing siRNA-mediated silencing of <i>Myb</i> or <i>Cebpa</i> for 20 hours. (B) Grouping four independent experiments, boxplots depict the variations in percentage of FLT3⁺ cells within the KSL populations. (C) Quantitative PCR analysis of <i>Myb</i> and <i>Cebpa</i> RNA expression in sorted transfected KSL cells 20 hours post transfection. Expression is normalised to <i>β</i>_<i>2</i><i>-microglobulin</i> and standardised to the control samples. Error bars represent the standard error of the mean. Plots are representative of 4 independent experiments. Numbers are plotted as mean ± SEM (**, p<0.001 and *, p<0.05).</p