Impaired ICM outgrowth of <i>Med28</i>^<i>-/-</i> blastocysts.

Abstract

<p><b>(A)</b> Representative PCR genotyping of E3.5 blastocysts from <i>Med28</i>^<i>+/-</i> intercrosses show WT (+) allele and null (-) allele. Primers described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0140192#pone.0140192.g001" target="_blank">Fig 1C</a> are used for genotyping. <b>(B)</b> Phase contrast microscopy images of embryos from <i>Med28</i>^<i>+/-</i> intercrosses. At embryonic day E3.5, <i>Med28</i>^<i>+/+</i> blastocysts (panel A; a, enlarged inset) are indistinguishable from <i>Med28</i>^<i>-/-</i> blastocysts (panel B; b, enlarged inset). After 3 days in culture (DIV3), unlike <i>Med28</i>^<i>+/+</i> blastocysts that show outgrowth (panel C, arrow), ICMs from <i>Med28</i>^<i>-/-</i> blastocysts fail to show outgrowth (panel D). A total of 14 WT and 12 mutant blastocysts were examined. Scale bar, 100 μm.</p