Expression of PDL1 on PC and monocytes in myeloma bone marrow.

Abstract

<p>(A) PDL1 on plasma cells: Bone marrow cells were stained with antibodies against CD45, CD138, CD38, CD19, and CD274 (PDL1). Gates were set on FSC and SSC and doublets and CD19+ cells were excluded. Gating strategy is shown in Fig A in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0139867#pone.0139867.s002" target="_blank">S2 File</a>. The distribution of % PDL1⁺ PC in the bone marrow of patients (n = 14) is shown. (B) Proportion of PDL1⁺ PC does not increase with tumor load. The % PDL1⁺ gated CD38⁺CD19^- PC versus % bone marrow plasma cells is plotted. Each dot represents one patient. P values were calculated from a Spearman’s test (n = 14). (C) PDL1 on monocytes and DCs: Bone marrow cells were stained with antibodies against lineage (CD3, CD19, CD56, CD138, CD15, CD34, and CD235a), CD45, HLADR, and CD11c. The gating strategy is shown in Supplementary S1B Fig. Gates were set on FSC and SSC, doublets excluded, and gates further set on lineage- CD45⁺cells. Figure shows distribution of % PDL1+ monocytes/DC in the bone marrow of patients (n = 14). (D) Correlation of % PDL1+ PC and monocytes/DC; % PDL1⁺CD11c⁺DR⁺ monocytes/DC versus % PDL1⁺CD38⁺CD19^- plasma cells is plotted. Each dot represents one patient. P value was calculated from a Spearman’s test.</p