<p>Monolayers of HMVEC were stimulated with D<sub>3</sub> (10 μM), 7-DHC(10 μM), or 0.5% DMSO (vehicle control) in the presence of inflammatory cytokines: IL-1β (10 ng/mL), TNF-α (2 ng/mL), and LPS (100 ng/mL) in an (<b>A-C</b>) ECIS or (<b>D</b>) transwell leak assay. (<b>E</b>) VEGF-induced leak of a fluorescent reporter in arterioles isolated from wild-type mice fed either standard chow or a D<sub>3</sub>-enhanced chow. All panels depict mean ± SEM. * denotes P<0.05, ** denotes P<0.01, and **** denotes P<0.0001.</p

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