Effect of PNAP-1, -3h, and -6h on MCF-7 cell cycle progression.

Abstract

<p>MCF-7 cells were treated with <b>PNAP</b>-<b>1</b>, -<b>3h</b>, and -<b>6h</b> at three concentrations (5, 10, and 25 μM) for 48 h, and then, (A) the cells were fixed and stained with propidium iodide to analyze the DNA content by flow cytometry. (B, C) Cell cycle-associated proteins (cyclin D1, CDK4, cyclin E, CDK2, p21, p27, cyclin B1, and CDK1) were detected and analyzed by western blot. The expression was quantified with the computerized Gel-Pro Analyzer image analysis system. The data are expressed as the means ± SEM from three independent assays. *P<0.05 and **P<0.01 are significantly different from the control.</p