Enhanced effects of cisplatin, 5-FU or clove oil on HCT16 cells at 39°C.

Abstract

<p><b>Top panel</b>: About 20,000 HCT116 cells were seeded in a 35-mm dish and allowed to grow for 3 days at 37°C or 39°C. The cells were then treated with indicated agent for 3 days, followed by staining the viable cells with crystal violet. <b>Bottom panel</b>: The cells were treated as above but were allowed to grow for 3 more days after the 3-day treatment with the indicated agent was terminated. Note that the difference in cell density between 37°C and 39°C was widened for cisplatin- and clove-treated cells, compared with the counterpart at the top panel. However, this post-treatment effect is not discerned in 5-FU treated cells at 39°C.</p

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