Confirmation of microarray results by sqRT-PCR.

Abstract

<p>Beta-actin (<i>Bact</i>), a housekeeping gene, was used to normalize gene expression in mouse CH, RPE and PR. The light blue bars indicate expression levels in CH, the blue bars expression levels in the RPE and the dark blue bars indicate expression levels in PR. Similar to the microarray data the expression level is highest in the RPE and lowest in the PR. The sqRT-PCR results confirm our findings; however <i>Tshr</i> and <i>Slc16a8</i> show expression lower in RPE compared to choroid. Overall, the sqRT-PCR confirmation rate in this, and in all our previous studies (combined), using exactly the same methodology and platform to investigate neuroepithelia from human donor eyes and brains was 87% [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0141597#pone.0141597.ref012" target="_blank">12</a>–<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0141597#pone.0141597.ref014" target="_blank">14</a>].</p