<p>(<b>A</b>) Schematic of the <i>eut</i> operon. (<b>B</b>) <i>In vitro</i> growth curve of S. Typhimurium WT (SL1344), Δ<i>eutR</i> (CJA009), or Δ<i>eutB</i> (CJA020) strains in LB without or with supplementation of 5 mM ethanolamine (EA). Each data point shows the average of three independent experiments. (<b>C</b>) qRT-PCR of <i>eutR</i> in WT or the Δ<i>eutB</i> (CJA020) <i>S</i>. Typhimurium strains grown in Dulbecco’s Modified Eagle Medium (DMEM) or DMEM supplemented with 5 mM EA. n = 3; error bars represent the geometric mean ± standard deviation (SD); <i>strB</i> was used as the endogenous control. (<b>D-F</b>) Competition assays between (<b>D</b>) Δ<i>eutB</i>::Cm<sup>R</sup> (CJA018) and WT strains; (<b>E</b>) Δ<i>eutR</i>::Cm<sup>R</sup> (CJA007) and WT strains; or (<b>F</b>) Δ<i>eutR</i>::Cm<sup>R</sup> (CJA007) and Δ<i>eutB</i> (CJA020) strains. Mice were orogastrically inoculated with 1:1 mixtures of indicated strains. Colony forming units (cfu) were determined at indicated time points. Each bar represents a competition index (CI). Horizontal lines represent the geometric mean value ± standard error (SE) for each group (n = 2 litters (6–8 animals)). *, <i>P</i> ≤0.05; **, <i>P</i> ≤ 0.005; ***, <i>P</i> ≤0.0005; <i>P</i> > 0.05 = ns.</p
