Effect of mutations in NS1 on the production of infectious DENV particles.

Abstract

<p>(<b>A</b>) <i>Released infectivity from DVR2A NS1 mutant-transfected cells</i>. VeroE6 cells were electroporated with DENV genomes containing NS1 mutations specified at the bottom. Seventy-two hours post-electroporation, supernatants containing infectious virus were used to infect naïve VeroE6 cells and luciferase activity measured 48 h later (black columns). Luciferase activity in the lysates 72 h post-electroporation reflecting replication is also shown (white columns). The background of the assay is determined with the active site NS5 polymerase mutant (GND). Mean values and standard deviations of three independent experiments are shown. Colours at the top of the panels refer to NS1 protein domains as described in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005277#ppat.1005277.g002" target="_blank">Fig 2A</a>. (<b>B</b>) <i>Efficiency of virus production relative to replication fitness</i>. To calculate specific defects in infectious particle production, data presented in (A) are shown as ratio of released infectivity/viral replication, and expressed as fold of wild-type (WT). Note that only NS1 mutants with viral RNA replication above input values were considered (cf. <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005277#ppat.1005277.g002" target="_blank">Fig 2B</a>; replication > Log₁₀0).</p