Knocking down of TRIM59 decreased the expression of cell cycle proteins.

Abstract

<p>H1299 cells transiently transfected with TRIM59 siRNA-1, TRIM59 siRNA-2 or control siRNA were cultured in RPMI 1640 with 10% FBS for 48 hrs. <b>(A)</b> The mRNA expression levels of G2/M phase related genes CDC2, CCNA1, CCNB1, CCNB2 and CDC25C were tested by quantitative Real-Time PCR. <b>(B)</b> The protein expression levels of CDK1 (also known as CDC2), CDC25C and cyclin B1 were checked by western blot with indicated antibodies.</p