PEF variation over the metaphase plate impacts oscillation amplitude and deviation from alignment.

Abstract

<p>(A) Average absolute PEF (on sister 1) binned by distance <i>r</i> from the centre of the metaphase plate (bins of approximately equal number; alternating grey and white boxes). (B) Average profile of the proportion of the opposing force (spring + PEF) to <i>F</i>₋ on lead sister during a LIDS for trajectories with <i>r</i> ≥ 4 μm (cyan) and <i>r</i> ≤ 3 μm (blue). Switch events aligned as described under <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1004607#pcbi.1004607.g008" target="_blank">Fig 8</a>. Solid and dashed lines indicate mean and ±s.e.m., respectively. (C-F) Trajectories binned by distance <i>r</i> as in (A) with bin mean of (C) alignment deviation; (D) oscillation amplitude; (E) metaphase plate thickness; (F) inter-sister distance shown. Lines in (D,F) indicate s.d., <i>n</i> = 843, see text for definitions.</p