Induction of <i>S</i>. <i>pombe</i> homologs of DJ-1.
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Abstract
<p>(A) qRT-PCR analysis of expression of <i>S</i>. <i>pombe hsp3101-hsp3105</i> and <i>sdj1</i> genes in the wild-type cells. Total RNA was isolated from the wild-type cells grown in YES medium at the indicated time points. All mRNA levels were normalized to the control <i>act1</i><sup>+</sup> mRNA level and were expressed as fold change relative to the mRNA levels at the 7 h time point, which was set at a value of 1. Data are presented as mean ± SD (<i>p</i> ≤0.01; <i>t</i> test). (B) Immunoblot analyses of Hsp3101, Hsp3102, Hsp3105 and Sdj1 expression in wild-type cells. Crude extracts were prepared from the wild-type cells at indicated time points (h). Total proteins were separated on SDS/PAGE gels and immunoblotted using anti-Hsp3101 Ab, anti- Sdj1 Ab, anti-Myc Ab, which detects Hsp3102-Myc and Hsp3105-Myc, and anti-Sla1 Ab (serves as a loading control).</p