ROS level and expression of NADPH oxidase were elevated in HCC.

Abstract

<p>(A) Fresh human HCC tissues and adjacent noncancerous tissues were stained with DHE. The fluorescence signals were captured by fluorescent microscopy and analyzed by Image J software. (B) Malondialdehyde content in human liver tissue was measured by thiobarbituric acid assay. (C) Activity of superoxide dismutase and glutathione peroxidase was measured by glutathione assay. (D) Expression of NADPH oxidase subunit p47phox, NOX2, p40phox and p67phox was measured by western blotting and normalized against GADPH. The results are presented as mean ± SEM. * indicates significant difference compared to adjacent liver tissues, <i>P</i><0.05.</p