ROS accelerated G1/S transition and increased cyclin E/CDK2 expression in WB-F344 cells.

Abstract

<p>(A) Cell cycle was assessed by flow cytometry. After starvation, cells were treated with UII (10⁻⁹ M) for 12 h, with or without apocynin (0.5 mM) pre-treatment for 30 min. The results are presented as mean ± SEM (n = 5). * <i>P</i><0.05 versus control. # <i>P</i><0.05 versus UII group. (B) Expression of cyclin E and CDK2 was examined by western blotting and normalized against GADPH. Data are presented as mean ± SEM (n = 3). * <i>P</i><0.05 versus control. # <i>P</i><0.05 versus UII group.</p