Experiments on using acclimated culture were carried out to determine if compounds
other than glucose could be effective as cosubstrate in biodecolourisation of an azo
dye. The role of cosubstrate was found to be not structure dependent hence
cometabolism was not likely to be involved in biodecolourisation. This finding also
offers opportunities for use of carbon sources other than glucose and for mixing of
wastewaters to facilitate biodecolourisatio