Rescue of <i>cart</i>^<i>1</i> phenotype by expressing human OCT2 in photoreceptors.

Abstract

<p>(A) OCT2 was able to transport carcinine. S2 cells transiently expressed OCT2-mCherry in a culture medium to which carcinine was added at a final concentration of 20μM or 100 μM. Cells were immunolabeled with anti-carcinine (green), and the mCherry (red) signal was observed directly. The carcinine signal is stronger in the presence of 100μM carcinine. (B-D) ERG recordings from (B) wt: <i>w</i>^<i>1118</i>, (C) <i>cart</i>^<i>1</i>, and (D) <i>cart</i>^<i>1</i>; <i>PninaE-oct2</i> flies showed restored “on” and “off” transients after photoreceptor cell-specific expression of human OCT2 in <i>cart</i>^<i>1</i> flies. (E) Quantification of phototactic behaviors of wt, <i>cart</i>^<i>1</i>, and <i>cart</i>^<i>1</i>; <i>PninaE-oct2</i> (<i>cart</i>^<i>1</i>; <i>oct2</i>) flies. Error bars represent SD. Significant differences between mutant and wt flies were determined using unpaired <i>t</i>-tests (*p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant).</p