Controllable expression of <i>osterix</i>, <i>BSP</i> and <i>osteocalcin</i> in MSCs-<i>TetR</i>/<i>Amelx</i> by Dox treatment.

Abstract

<p>MSCs-<i>TetR</i>/<i>Amelx</i> were cultured in the osteogenic induction medium in the presence (+) (black bars) or absence (-) (white bars) of Dox for 17 days in four different conditions. (Condition I: day 0–17 Dox-; Condition II: day 0–14 Dox-, day 15–17 Dox+; Condition III: day 0–14 Dox+, day 15–17 Dox-; Condition IV: day 0–17 Dox+). Expression of <i>Runx2</i> (<b>a</b>), <i>osterix</i> (<b>b</b>), <i>BSP</i> (<b>c</b>), <i>osteopontin</i> (<b>d</b>) and <i>osteocalcin</i> (<b>e</b>) was determined by a quantitative real-time RT-PCR analysis. Significant differences (**<i>P</i><0.01, *<i>P</i><0.05: ANOVA with Tukey’s multiple comparison test: n = 3) within each condition are shown.</p