Effects of forced expression of <i>Amelx</i> on ALP activity and mineralized nodule formation.

Abstract

<p>MSCs-<i>TetR</i>/<i>Amelx</i> at 3 passages were cultured in the growth medium (Con) or osteogenic induction medium (Os) in the presence (<b>+</b>) or absence (-) of Dox in 24-well plates. (<b>a</b>) ALP activity on day 7 and 14 was examined by ALP staining (bars: 100 μm). (<b>b</b>) Mineralized nodule formation on day 21, 28 and 35 was detected by von Kossa staining. (<b>c, d</b>): Calcium deposition was determined by Alizarin Red S staining on day 7, 14 and 21 (<b>c</b>) and the staining intensity was quantitatively analyzed (<b>d</b>). Significant differences (*<i>P</i><0.01: ANOVA with Tukey’s multiple comparison test: n = 9) are shown.</p