PbeIF2α-P phosphatase activity of UIS2.

Abstract

<p>(A) PbUIS2 phosphatase domain (PD) dephosphorylated eIF2α-P i<i>n vitro</i>. PfeIF2α-P[γ-³²P] was incubated with GST-PbUIS2PD in the presence of 5mM MnCl₂. (B) Characterization of PbUIS2 as a PP2C/PPM phosphatase. PbUIS2PD phosphatase activity was measured by malachite green colorimetric assay in the presence of EDTA, okadaic acid (OA), or CdCl₂. E: GST-PbUIS2PD; S: eIF2α-P. The inorganic phosphate was detected with malachite green by measuring the absorbance at 630 nm. Each value is the mean ± SD of three independent experiments.</p