<i>H</i>. <i>pylori</i> infection promotes the genes and proteins expression in gastric cancer tissues.

Abstract

<p>(A) Western blot analysis of the indicated proteins in the control cells (1), <i>H</i>.<i>pylori</i>-infected SGC-7901 cells (2) and <i>cagA</i>-overexpressed SGC-7901 cells (3). GAPDH served as the loading control. The data are representative of three independent experiments. (B) Quantitative RT-PCR analysis of the indicated genes in 30 gastric cancer tissues. Values are represented as average Ct fold compared to peri-cancerous tissues, where peri-cancerous tissues were set to 1. (C) Western blot analysis of the indicated proteins in 30 gastric cancer tissues. 200 mg tissues were homogenized and total proteins were collected. A total of 50 μg protein extracts were subjected to SDS-PAGE gel electrophoresis. GAPDH served as the loading control. (D) Detection of the <i>H</i>. <i>pylori</i> 16S <i>rRNA</i> gene and <i>cagA</i> gene in gastric cancer tissues by PCR (Upper). M represents the DNA molecular weight marker. Lane 1 is the positive control. Lane 2 is the negative control. Lanes 3, 4 and 6 are positive samples. Lanes 5 are negative samples. Quantitative RT-PCR analysis of the indicated genes in gastric cancer tissues with and without <i>H</i>. <i>pylori</i> infection (Lower). Values are presented as the average Ct fold compared to the group without <i>H</i>. <i>pylori</i> infection, which was set to 1. The figure presents the average of 30 samples. Data are presented as the means ± SD. Error bars represent standard deviations. LDH, L-lactate dehydrogenase. DLD, Dihydrolipoamide dehydrogenase. PRPF19, pre-mRNA processing factor 19 homolog. RanGAP, Ran-specific GTPase-activating protein. CaM, calmodulin. p64 CLCP, nuclear chloride ion channel protein. *, <i>P</i><0.05 compared to peri-cancerous tissue (B and C) and tissues without <i>H</i>. <i>pylori</i> infection (D).</p