Introduction of CagA into gastric cancer cells.

Abstract

<p>(A and B) Western blot analysis of CagA and phosphorylated CagA in <i>H</i>. <i>pylori</i>-infected SGC-7901(A) and AGS (B) cells. The cells infected with the indicated ratio of cells to <i>H</i>. <i>pylori</i> for the indicated time were collected and lysed, and the proteins were separated by SDS-PAGE. Cells infected with <i>H</i>. <i>pylori</i> boiled for 15 min at a MOI of 1:1000 were used as a control. (C and D) Detection of CagA mRNA and protein in <i>cagA</i>-overexpressing SGC-7901 cells by RT-PCR (C) and western blot (D). GAPDH served as the loading control. The data are representative of three independent experiments. <i>Hp</i>, <i>H</i>. <i>pylori</i>; P-CagA, phosphorylated CagA; GAPDH, Glyceraldehyde-3-phosphate- dehydrogenase.</p