Representative 2-DE maps and magnified image of differential spots in three cell lines.

Abstract

<p>SGC-7901 and AGS cells infected with <i>H</i>. <i>pylori</i> for 6 h at a MOI of 1:1000 (cell to <i>H</i>. <i>pylori</i>) and SGC-7901 cells transfected with pcDNA3.1/<i>cagA</i> for 48 h were collected and lysed, and the protein concentrations were determined using Bradford colorimetry. A total of 800 μg of protein was loaded for two-dimensional electrophoresis. Cells infected with boiled <i>H</i>. <i>pylori</i> or transfected with empty vector served as controls for the infected or transfected cells, respectively. (A) SGC-7901 cells infected with <i>H</i>. <i>pylori</i>. (B) AGS cells infected with <i>H</i>. <i>pylori</i>. (C) SGC-7901 cells transfected with the <i>cagA</i>-vector. (D) Magnified image of 10 differential spots. B4, B11, C6, C7, C8 and C9 spots were up-regulated, whereas D12, D16, E2 and E11 spots were down-regulated. These spots are identified in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0146521#pone.0146521.t003" target="_blank">Table 3</a>.</p