<p>Immunized mice were challenged 4 times with OVA or saline, as shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0146686#pone.0146686.g002" target="_blank">Fig 2</a>. Anti-CD4 mAb or control rat IgG was administered twice, that is, at 9 and 6 days before the last challenge. Six hours after the last challenge, the CD3<sup>+</sup>CD4<sup>+</sup> population in the spleen was determined by flow cytometry (A). Representative data from 3 independent experiments are shown. The number of sneezes evoked by histamine (B) and the accumulation of lymphocytes, neutrophils, and eosinophils in NALF (C) were also examined. Data are expressed as mean ± SEM for 4–6 animals. *<i>p</i> < 0.05, **<i>p</i> < 0.01, ***<i>p</i> < 0.01, compared with OVA-challenged control mice (Dunn’s test).</p
