<p>Immunoblot analyses (A-H). The protein level of phosphorylated and activated mTOR (pmTOR) was not increased in the retina 3 hours after the induction of inflammation by an intraperitoneal lipopolysaccharide (LPS) injection, although the level of pmTOR was suppressed by the rapamycin treatment (A, B). At 12 hours after the LPS injection, the pmTOR expression was increased in the retina, which was suppressed by the rapamycin treatment (C, D). The pmTOR level in the retina at 12 hours was also suppressed by the NF-κB inhibitor, DHMEQ (E, F). The ratio of the LC3II to LC3I protein levels (an autophagy marker) in the retina was not changed after LPS injection but was increased by rapamycin at 12 hours after LPS injection (G, H); n = 5. *p < 0.05, **p < 0.01.</p
