FISH of canine endoscopic biopsies.

Abstract

<p>Triple color FISH identifies bacterial organisms within different mucosal compartments of endoscopic ileal and colonic biopsies obtained from healthy dogs. Panel A = colon biopsy hybridized with Cy3-Ebac1790; Panel B = colon biopsy hybridized with Cy3-Bac303; Panel C = ileum hybridized with Cy3-Ebac1790; and Panel D = ileum hybridized with Cy3-Ec (<i>E</i>. <i>coli</i>). All other bacteria that hybridize exclusively with the universal probe (Eub338-FITC) appear green. DAPI-stained colonic mucosa with goblet cells appears blue. FM = free mucus; AM = adherent mucus.</p