PCR analysis for EBV genotyping for 1/2,F/f,C/D.

Abstract

<p><b>Lanes 1–8:</b> Lane M, DL 2000 DNA Marker; lane 1, negative control; lane 2, B95-8 prototype (Type 1); lane 5,7,8, representative cases of Type 1; lane 3,4,6,representative case of co-infection with Type 1 and 2; no subtype 2 was detected in the study. <b>Lanes 9–16:RFLP analysis with BamHI restriction enzyme digestion after PCR amplification at BamHI W1/I1 region.</b> Lane M, DL2000 DNA Marker; lane 9, negative control; lane 10, Raji prototype (Type D); lane 11, 13 and 15, representative cases of Type C strains; lane 12,14,16, representative cases of Type D strains. <b>Lanes 17–24: RFLP analysis with BamHI restriction enzyme digestion after PCR amplification at BamHI F region.</b> Lane M, DL2000 DNA Marker; lane 17, negative control; lane 18, B95-8 prototype (Type F); lane 19–24 representative cases of the Type F strains; No type f strain was found in the present study.</p