Temporal expression of housekeeping genes.

Abstract

<p>Raw Ct values for the mRNA of seven <i>A</i>. <i>americanum</i> housekeeping genes from tick salivary glands (A) and midgut (B) throughout a blood meal (unfed–168 h). Each gene was amplified from each sample and the point at which the amplification curve crosses the threshold is considered the Ct.</p