Gal3 expression in DRGs is increased after SNL.

Abstract

<p>(A) The mRNA level of gal3 is increased in DRGs of rats subjected to L5 SNL. Total RNA was extracted from the fifth lumbar dorsal horn sections and was subjected to real-time PCR to analyze the relative expression level of gal3 in each sample. Each sample was analyzed in triplicate. The 2^-ΔΔCt method was used to quantify the relative levels of gal3. β-actin was used as reference for mRNA. n = 10. *<i>p</i>< 0.05 vs sham, # <i>p</i>< 0.05 vs SNL group. (B and C) Western blot analysis of gal3 protein expression in the fifth lumbar dorsal horn sections in each sample.*<i>p</i>< 0.05 vs sham, # <i>p</i>< 0.05 vs SNL group.</p