Comparative Proteomic and Metabolomic Analysis of Staphylococcus warneri SG1 Cultured in the Presence
and Absence of Butanol
- Publication date
- Publisher
Abstract
The complete genome of the solvent
tolerant Staphylococcus
warneri SG1 was recently published. This Gram-positive
bacterium is tolerant to a large spectrum of organic solvents including
short-chain alcohols, alkanes, esters and cyclic aromatic compounds.
In this study, we applied a two-dimensional liquid chromatography
(2D-LC) mass spectrometry (MS) shotgun approach, in combination with
quantitative 2-MEGA (dimethylation after guanidination) isotopic labeling,
to compare the proteomes of SG1 grown under butanol-free and butanol-challenged
conditions. In total, 1585 unique proteins (representing 65% of the
predicted open reading frames) were identified, covering all major
metabolic pathways. Of the 967 quantifiable proteins by 2-MEGA labeling,
260 were differentially expressed by at least 1.5-fold. These proteins
are involved in energy metabolism, oxidative stress response, lipid
and cell envelope biogenesis, or have chaperone functions. We also
applied differential isotope labeling LC-MS to probe metabolite changes
in key metabolic pathways upon butanol stress. This is the first comprehensive
proteomic and metabolomic study of S. warneri SG1 and presents an important step toward understanding its physiology
and mechanism of solvent tolerance