Facile Method for Modification of the Silicon Nanowires
and Its Application in Fabrication of pH-Sensitive Chips
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Abstract
A novel, facile, and effective method
for modification of SiNWs or SiNW arrays has been developed. In this
method, reaction between reductive Si–H bonds on the surface
of SiNWs and the aldehyde group containing in organic molecules has
been used for immobilization of organic molecules onto the surface
of SiNW arrays. The method is time saving and can be operated at room
temperature without any other complex reaction requirement. Fluorescence
images, XPS, fluorescence spectra, and IR spectra were used for characterization
of the modification. Through this method, a SiNW array-based pH sensitive
chip was realized by covalently immobilizing 5-aminofluorescein molecules
onto the surface of SiNW arrays with glutaraldehyde as linker molecules.
Fluorescence intensity of the chip increased with increasing of pH
value and a linear relationship between fluorescence intensity and
pH values was acquired. In addition, the chip has been successfully
used for real-time and in situ monitoring of extracellular pH changes
for live HeLa cells and the result exhibited fine resolution of time
and space
