DNA-Grafted Polypeptide
Molecular Bottlebrush Prepared
via Ring-Opening Polymerization and Click Chemistry
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Abstract
A new type of DNA grafted polypeptide molecular brush
was synthesized
via a combination of ring-opening polymerization (ROP) and click chemistry.
This conjugation method provides an easy and efficient approach to
obtain a hybrid DNA-grafted polypeptide molecular bottlebrush. The
structure and assembly behaviors of this hybrid brush were investigated
using electrophoresis, UV–vis spectroscopy, transmission electron
microscopy (TEM), and atomic force microscopy (AFM). Hierarchical
supramolecular assemblies can be obtained through hybridization of
two kinds of polypeptide-<i>g</i>-DNA molecular bottlebrushes
containing complementary DNA side chains. We further demonstrated
that such polypeptide-<i>g</i>-DNA can be hybridized with
ds-DNA and DNA-grafted gold nanoparticles to form a supermolecular
bottlebrush and hybrid bottlebrush, respectively. In addition, DNA-polypeptide
hydrogel can be prepared by hybridization of polypeptide-<i>g</i>-DNA with a linker-ds-DNA, which contains the complementary “sticky
ends” to serve as cross-linkers