Plant cytokinin signaling is differently affected during <i>cks1</i> infection.

Abstract

<p>The transcriptional regulation of CK marker genes (<i>OsRR6</i> (Os04g57720) and <i>OsRR1</i> (Os11g04720) as named by Pareek et al., (2006)[<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005457#ppat.1005457.ref053" target="_blank">53</a>] was evaluated by quantitative RT-PCR using the Actin gene for normalization. Nipponbare plants were inoculated with spore suspension (in gelatin 0.5%) of either the <i>cks1</i> mutant (black bars) or <i>cks1</i>^<i>CKS1</i> control strain (white bars) and gene expression was measured at 2, 4 and 6 hours post inoculation (hpi), before penetration of the leaf tissues but at stages where there was no significant difference in growth of the <i>cks1</i> mutant compared to the complemented strain (<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005457#ppat.1005457.s005" target="_blank">S3C Fig</a>). The values presented are the Log2 ratios (infected/not infected) of the means calculated from four independent replicates. Uninfected plants were sprayed with gelatin 0.5% but without spore suspension. This experiment was repeated three times and showed similar results. A t-test was used to compare the means of expression quantified in <i>cks1</i> (black bars) and <i>cks1</i>^<i>CKS1</i> (white bars) inoculated plants. *: values significantly different at p-value < 0.05.</p