Antigen presenting cells from aged mice do not upregulate MHC class II expression in response to vaccine components <i>in vitro</i>.

Abstract

<p>Bone marrow derived macrophages (BMM) or dendritic cells (BMDC) from young adult (6–8 weeks, dark bars) and aged (18–24 months, light bars) were stimulated <i>in vitro</i> with CpG, Fluzone (FZ) or both (FZ+CpG) overnight. Up regulation of MHC class II relative to unstimulated cells (media) was measured using flow cytometry on (A) BMM (F4/80+/CD11b+) and (C) BMDC (CD11c+/CD11b^med /F4/80-). TNF-α production was measured by ELISA in supernatants from (B) BMM and (D) BMDC. Readings were taken as an average of triplicate wells. Results shown are mean values + S.D and are representative of 2 independent experiments.</p