<p>(a) Sleep profiles of <i>norpA</i> males following 4 days of pair-wise social interaction in 12:12 h light/dark cycles (LD12:12). (b) Sleep profiles of <i>CS</i> and <i>Iso31</i> males following pair-wise social interaction for 4 days in constant darkness (DD). (c) Sleep analysis revealed that daytime sleep is significantly increased in socialized <i>norpA</i> males as compared to solitary controls (<i>p <</i> 0.0005 for daytime and <i>p <</i> 0.02 for nighttime, Student’s <i>t</i>-test; <i>n</i> = 21 and 24 for socialized and control groups respectively). Daytime sleep of socialized <i>CS</i> (<i>p <</i> 0.02, Student’s <i>t</i>-test; <i>n</i> = 14 and 16 for socialized and control groups respectively) and <i>Iso31</i> (<i>p <</i> 0.01, Student’s <i>t</i>-test; <i>n</i> = 29 and 18 for socialized and control groups respectively) males is also significantly increased as compared to that of solitary controls. (d, left) Sleep profiles and (d, right) change in sleep of <i>Gr66aGAL4>UAShid</i> and <i>Gr33aGAL4>UASdti</i> males following 4 days of pair-wise social interaction. Daytime sleep of socialized <i>Gr66aGAL4>UAShid</i> males (<i>p <</i> 0.0001, Student’s <i>t</i>-test; <i>n</i> = 16 and 14 for socialized and control groups respectively) and socialized <i>Gr33aGAL4>UASdti</i> males (<i>p <</i> 0.0001, Student’s <i>t</i>-test; <i>n</i> = 32 and 19 for socialized and control groups respectively) is significantly increased in comparison to that of solitary controls. Nighttime sleep of <i>Gr33aGAL4>UASdti</i> males is also significantly increased (<i>p <</i> 0.0005, Student’s <i>t</i>-test) in comparison to that of that of solitary controls. (e, left) Sleep profiles and (e, right) change in sleep of <i>Orco</i> males following 4 days of pair-wise social interaction. Sleep analysis revealed that day as well as nighttime sleep of socialized <i>Orco</i> males is comparable (<i>p ></i> 0.05; <i>n</i> = 27 and 20 for socialized and control groups respectively) to solitary controls, whereas daytime sleep of socialized <i>Iso31</i> males is significantly greater as compared to that of solitary controls (<i>p <</i> 0.05; <i>n</i> = 13 and 10 for socialized and control groups respectively), daytime sleep-enhancement in <i>Iso31</i> flies is significantly greater (<i>p <</i> 0.05) than that of <i>Orco</i> flies. (f, left) Sleep profiles and (f, right) change in sleep of <i>Or83bGAL4>UASKir2</i>.<i>1</i> males following 4 days of pair-wise social interaction. (f, right) Day as well as nighttime sleep of socialized <i>Or83bGAL4>UASKir2</i>.<i>1</i> males is comparable (<i>p ></i> 0.05) to that of solitary controls (e, right), whereas socialized parental (<i>Or83bGAL4/+</i>, <i>p <</i> 0.05 and <i>UASKir2</i>.<i>1/+</i>, <i>p <</i> 0.005; <i>n</i> = 13–16 per group per genotype) males show a statistically significant increase in sleep as compared to solitary controls. Daytime sleep-enhancement in parental control flies is significantly greater (<i>p <</i> 0.0001 for both) than that in silenced flies. Horizontal lines with asterisks above a pair of bars indicate statistically significant difference in sleep-enhanced in the experimental versus control genotypes. Other details are same as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0150596#pone.0150596.g001" target="_blank">Fig 1</a>.</p
