Expression of <i>SLC45A3-ELK4</i> and <i>CTCF</i>, and CTCF binding to the two insulators in different cell lines.

Abstract

<p>Hela (cervical cancer cells), 293T (SV40 transformed embryonic kidney cells), HCT116 (colon cancer cells), LNCaP, and RWPE-1 (prostate cells). (A) <i>SLC45A3-ELK4</i> expression level was measured by qRT-PCR, normalized against <i>GAPDH</i>, and further normalized against the level in LNCaP. (B) <i>CTCF</i> expression level measured by qRT-PCR, normalized against <i>GAPDH</i>, and further normalized against the level in LNCaP. (C) Binding of CTCF to the two insulators measured by ChIP and qPCR. IgG was used as control for the CTCF antibody.</p