Phylogenetic tree constructed using a ML method from the non-recombinant NFLG sequences of 149 blood donor samples, 53 non-redundant B, eight subclade F1, and eight subtype C sequences from Brazil retrieved from GenBank database.
<p>Besides these sequences, the tree also included 37 reference strains (Los Alamos database) representing subtypes A-D, F-H, J and K. Sequences identified in this study are indicated by circles and the geographic origin of each sequence is color-coded. Monophyletic clusters from the same geographic origin are indicated by thick green branches. For clarity purposes, the tree was midpoint rooted. Gray arrows indicate hypermutated sequences with a predominance of unidirectional G-to-A mutations (010BR_MG005, and 010BR_SP022). The aLRT values of at least 80% are indicated at nodes. The scale bar represents 0.05 nucleotide substitutions per site.</p
