Effects of <i>FgPRP4</i> deletion on intron splicing.

Abstract

<p>(<b>A).</b> Box-plot comparison of intron retention levels between the wild type (PH-1) and <i>Fgprp4</i> mutant (FP1) in replica experiments. The statistical significance for each comparison is analyzed by <i>t</i>-test (****, <i>P</i><0.0001). (<b>B).</b> The percentage of introns and genes with the three marked intron retention levels in <i>Fgprp4</i> compared to the wild type. (<b>C)</b>. Introns that were increased in intron retention over 2-fold in <i>Fgprp4</i> in both replica experiments. (<b>D).</b> Intron splicing defects in the labelled genes were verified by RT-PCR with primers flanking the introns with reduced splicing efficiency (marked with *) in the <i>Fgprp4</i> mutant. Lanes 1–3 were PCR results with the genomic DNA, cDNA of PH-1, and cDNA of <i>Fgprp4</i>, respectively. The sizes of amplified bands are labelled on the side.</p