<i>Var</i> transcription profiles of parental parasite lines used for volunteer infection.

Abstract

<p>(A) The gene expression of each <i>var</i> gene and control genes relative to <i>sbp1</i> expression is shown in dot plots for the pre-mosquito Master Cell Bank (MCB) parasite line. Each point represents values observed per test generation for the MCB samples taken from two independently thawed parasite stocks after 6, 8 and 21 parasite generations, respectively (n = 6). The median expression per <i>var</i> gene is shown, housekeeping genes used as controls, <i>var</i> gene names and groups are indicated. (B) The MCB cell line exclusively expresses the group E <i>var2csa</i> gene as shown by the proportion of <i>var</i> transcripts according to <i>var</i> group affiliation. The genomic proportion of each <i>var</i> gene group is indicated after the color code.</p