Infection with RCAS-GFP retroviruses does not affect chick stomach development and differentiation. (A) Whole-mount in situ hybridization of E6 uninfected (control) and GFP-expressing gastrointestinal tracts using SM22, BAPX1 and SOX10 riboprobes. Scale bars, 1 mm. (B) Transverse sections of E7 control and GFP-expressing stomachs analysed either by in situ hybridization using the LIX1 riboprobe or by immunofluorescence with anti-αSMA and anti-CALPONIN antibodies. Nuclei were visualized with Hoechst. (C) Serial transverse sections of E7 control and GFP-expressing stomachs analysed by immunofluorescence using anti-PH3 antibodies. Nuclei were visualized with Hoechst. Graph represents the quantification of PH3-positive cells. ns, Not significant. (JPG 1115 kb
