Validation of PHASTpep software translation and frequency calculations.

Abstract

<p>(A) The raw data pulled from the fastq file of the Illumina sequencer showing the unique flanking regions (red) surrounding the portion of the DNA sequence corresponding to the displayed peptides (blue). (B) The unique flanking regions were used to isolate the peptide sequences, which were then translated into amino acids. For each sequence, a frequency was calculated corresponding to the number of times it appeared in the run. (C) GUI of the PHASTpep software presented in this paper to automate the data processing and analysis.</p