Additional file 2: Figure S1B. of 3D culture of Her2+ breast cancer cells promotes AKT to MAPK switching and a loss of therapeutic response

Abstract

Process of recovery of cells from 3D culture for experimental analysis. Cells were recovered from the 3D cultures for counting, immunocytochemical analysis or Western blotting as shown using a modification of a previously reported method (Arnold 2001). (DOC 52 kb