Effects of AKT and MAPK inhibition on apoptosis in BT474 and MDAMB361 cells. Cell lines grown in 2D or 3D culture were exposed to AKT inhibitor (MK-2206) or MEK inhibitor (U0126) prior to cell lysis and Western blotting using an antibody that recognizes full length (116kDa) and cleaved (85kDa) forms of PARP, the latter form apparent upon apoptosis. Neither AKT or MAPK inhibition resulted in a significant loss of full length PARP or a corresponding gain in cleaved PARP for either cell line. In contrast, cleaved PARP was seen to increase in response to the apoptosis inducer, camptothecin, included as a positive control. (DOC 59 kb
