<p>(A-B) <i>cln3</i> ATG MO morphants die prematurely. Progression of <i>cln3</i> ATG morphants demonstrated by monitoring cohorts of fish injected with 1.6 ng or 2.9 ng of <i>cln3</i> ATG MO and uninjected WT siblings. (A) The Kaplan-Meier survival curve shows that all WT larvae survive beyond 6 dpf, whereas the majority of morphants injected with 1.6 ng <i>cln3</i> ATG MO die between 3 and 6 dpf (median survival 5 dpf). Log rank (Mantel-Cox) test <i>p</i><0.0001 (<i>n</i> = 46 WT and <i>n</i> = 23 morphants). (B) The Kaplan-Meier survival curve shows that all WT larvae survive beyond 4 dpf, whereas the morphants injected with 2.9 ng <i>cln3</i> ATG MO die between 1 and 4 dpf (median survival 1 dpf). Log rank (Mantel-Cox) test <i>p</i><0.0001 (<i>n</i> = 16 WT and <i>n</i> = 31 morphants). Error bars indicate ± SE. (C-E) Increased activity was observed in <i>cln3</i> ATG MO morphants at 36 hpf. Analysis of number of spontaneous coils or tail flicks within a 3 minute time period comparing 1.6 ng <i>cln3</i> ATG MO morphants with age matched WT siblings was carried out at 24 hpf (C), 36 hpf (D) and 48 hpf (E). At 24 and 48 hpf the data show no significant difference in the number of coils or flicks. At 36 hpf the data show a significant difference in the number of flicks; <i>p</i><0.0001 (<i>n</i> = 10 zebrafish per treatment group). Data represent mean ± SD; results were evaluated using a 2-tailed unpaired Student’s <i>t</i>-test. (F-G) The <i>cln3</i> ATG MO morphant escape response is diminished. (F) The WT (control) fish aged 4 dpf respond to touch with a C-bend, a turn away from the stimulus and rapid swimming (0.03 second intervals). (G) 1.6 ng c<i>ln3</i> ATG MO morphants aged 4 dpf display a greatly attenuated escape response (0.06 second intervals).</p
