<p>A-N) <i>In situ</i> hybridisation for <i>cln3</i> (antisense) shows its expression from the one cell stage through to 4 dpf. From 1 dpf expression is increasingly restricted to the central nervous system. At 2 dpf <i>cln3</i> expression is particularly strong in the mid-hindbrain boundary (arrow) where the cerebellum is developing (G, H, M, N). (A’-L’) Experiments conducted using a <i>cln3</i> sense probe (sense) revealed the specificity of the antisense signal. Abbreviations: mhb, mid-hindbrain boundary.</p
