Abstract

<p>(A) Spotting analysis of αSyn, A30P, 4(Y/F) αSyn, A30P/4(Y/F) and GFP (control) expressed in <i>YHB1</i> and Δ<i>yhb1</i> yeast on non-inducing and galactose-inducing SC-Ura plates after 3 days of growth. (B) Cell growth analysis of <i>YHB1</i> and Δ<i>yhb1</i> yeast expressing αSyn, A30P, 4(Y/F), A30P/4(Y/F) and GFP (control) at time point 20 h. Significance of differences was calculated with t-test (*, <i>p</i> < 0.05; **, <i>p</i> < 0.01, n = 3). (C) Quantification of the percentage of cells displaying αSyn aggregates after 6 h induction in galactose-containing SC-Ura medium. Significance of differences was calculated with t-test (*, <i>p</i> < 0.05, **, <i>p</i> < 0.01, n = 6).</p

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